Genes in families where one or more members has only 1 exon and other members have more than one exon


January 25, 2002 © Mitrick A. Johns Dept. of Biological Sciences, Northern Illinois University


Background

Genes in these families have predicted peptides that match at least one other peptide in the famliy with a BLAST e-value of better than 10-180. Predicted genes come from the TIGR 08-10-2001 release (XML file). The predicted functions for these gene families are a concensus derived from the TIGR functional predictions for each of the genes.

Peptide and DNA sequences were aligned using the GCG (Wisconsin Package) "BestFit", "FrameAlign", and "PileUp" tools, with final adjustments made by hand. The aligned sequences can be seen for each family under the "Aligned DNA and peptide sequences" link.

Exon regions often contain short gaps that are not indicated here. Similarly, introns are often of different lengths, which are not indicated here. These drawings show the relative start and end positions of the coding sequences of the genes and the relative lengths of the aligned exons and introns.

Presence of ESTs for genes in the families

The Arabidopsis ESTs (Expressed Sequence Tags) from the August 2001 release containing 117,000 ESTs were each used as queries in a BLASTN search using the TIGR predicted genes as a database. The 5000 full length cDNAs from Ceres deposited with TIGR in March 2001 were also used. The gene with the best BLAST score was assumed to be the source of the EST. For each of the genes used in the gene families on this page, the presence of an EST that best matched the gene is given in the gene family's table. If the BLAST score was worse than 10-30, it is listed as a "weak" match.

EST confirmation of introns

If a gene contained introns, any corresponding EST or Ceres cDNA clones were examined to see if intron sequences had been removed. If evidence exists for all the introns, the table entry for the gene is "yes"; if only some introns are confirmed, the answer is given as "some"; if the EST sequences didn't cross the exon-intron boundaries, the answer was given as "no". It is important to note that "no" does not imply an experimental contradiction of the predicted introns, but instead it implies a lack of confirmatory evidence. In rare cases where an EST contradicted a predicted intron boundary, a correction was made in the diagram.

Genetic Distance between gene (DNA) sequences

As a simple and general measure of similarity, the genetic distance between each pair of family members was calculated. To compare the genes, the aligned sequences were trimmed at the 5' and 3' ends to a common length, and all intron sequences were removed. The resulting trimmed sequences can be seen under the "Exon sequences" link for each family. The exon sequences were then analyzed using the Jukes-Cantor distance method, the default with the GCG "Distances" program. This method calculates the number of mismatched bases, then applies a simple correction factor for multiple substitutions at a single site. Gaps are not penalized, and no distinction is made between transitions and transversions. Distances are expressed as the number of base substitutions per 100 bases). (Jukes T.H. and C. R. Kantor, 1969. Evolution of protein molecules. vol. 3, pp. 21-132. In H.N. Munro (ed.), Mammalian Protein Metabolism. Academic Press, New York)

The percentage of amino acids that are identical in the aligned peptide sequences was also calculated with the "Distances" program, using the uncorrected distance option. This gives the percentage of amino acids that differ between pairs of peptides, which was converted to percentage similarity by subtracting it from 100.

Synonymous vs. Non-synonymous substitution rates as an indicator of selection pressure

If two similar genes are both being subjected to selection pressure, due to the contribution of their protein products to the organism's fitness, non-synonymous base substitutions should be rarer than synonymous substitutions. Non-synonymous substitutions alter the amino acid sequence and potentially affect the protein's activity, and so they should be subjected to greater constraints than synonymous substitutions that don't change the protein sequence. Based on this prinicple, Li et al. (1985) developed a method that uses the ratio of synonymous to non-synonymous substitutions as evidence that a given portion of a gene has functional significance for the organism. This method, slightly modified, is the basis of the GCG "Diverge" program. It measures the corrected frequency of substitutions on aligned DNA sequences, and reports the ratio of synonymous to synonymous substitutions, Ks/Ka. A ratio greater than 1.0 implies that selection pressure exists for both genes. Thus, if both genes are active, the Ks/Ka ratio will be high, but if one or both genes is a pseudogene (selectively neutral), the ratio will be approximately 1.0. (Li, W.-H., C.-I. Wu, and C.-C. Luo. (1985) A new method for estimating synonymous and nonsynonymous rates of nucleotide substitution considering the relative likelihood of nucleotide and codon changes. Mol. Biol. Evol. 2:150-174.)

Dotplots

Dotplots provide a graphical comparison of 2 DNA or protein sequences. They provide a rapid visual method of seeing major structural differences between genes, such as the presence and absence of introns. It is not necessary to align the sequences in advance; dotplots can be used as an aid to producing the alignments. In the simplest conception, one sequence is plotted on the x-axis and the other sequence is plotted on the y-axis, and a dot is placed at each point of intersection if the two sequences match. This results in a diagonal line for the areas where the genes are similar. The noise level is reduced by using a sliding window, and placing a dot if a given number of matches occur within the window. Here, the "Dotplots" link shows the DNA sequences (showing introns) and the peptide sequences (without introns). Also, displayed are the similarites of the 500 bp upstream from the start of the coding sequence, and 500 bp downstream from the end of the coding sequences. The plots were produced using the GCG "Compare" and "Dotplot" programs. (Maizel, R.P. and Lenk, J.V. (1981) "Enhanced Graphic Matrix Analysis of Nucleic Acid and Protein Sequences" Proc. Natl. Acad. Sci. USA 78; 7665-7669 )

 


Gene Families


Gene family description for famliy 1653

ATP-dependent RNA helicase

EST Information
Gene ESTs exist? ESTs confirm introns?
at1g20960 yes no
at2g42270 yes --

Genetic distances | Percent peptide similarity (upper) and Ks/Ka ratios (lower)
-- at1g20960 at2g42270
at1g20960 -- 17.85 | 83.0%
at2g42270 5.068 --

Aligned DNA and peptide sequences

Exon sequences (used for distance and Ks/Ka calculations)

Dotplots for pairs of genes in the family, including DNA, peptides, 5' and 3' ends


Gene family description for famliy 1298

hypothetical protein

EST Information
Gene ESTs exist? ESTs confirm introns?
at1g31470 yes no
at3g01630 no --

Genetic distances | Percent peptide similarity (upper) and Ks/Ka ratios (lower)
-- at1g31470 at3g01630
at1g31470 -- 42.39 | 66.2%
at3g01630 5.291 --

Aligned DNA and peptide sequences

Exon sequences (used for distance and Ks/Ka calculations)

Dotplots for pairs of genes in the family, including DNA, peptides, 5' and 3' ends


Gene family description for famliy 1284

Putative protein

EST Information
Gene ESTs exist? ESTs confirm introns?
at5g40880 no --
at5g49200 no --

Genetic distances | Percent peptide similarity (upper) and Ks/Ka ratios (lower)
-- at5g40880 at5g49200
at5g40880 -- 13.94 | 79.8%
at5g49200 1.859 --

Aligned DNA and peptide sequences

Exon sequences (used for distance and Ks/Ka calculations)

Dotplots for pairs of genes in the family, including DNA, peptides, 5' and 3' ends


Gene family description for famliy 1130

Beta-glucan-elicitor receptor

EST Information
Gene ESTs exist? ESTs confirm introns?
at1g18310 no --
at5g15870 weak --

Genetic distances | Percent peptide similarity (upper) and Ks/Ka ratios (lower)
-- at1g18310 at5g15870
at1g18310 -- 26.55 | 79.0%
at5g15870 5.622 --

Aligned DNA and peptide sequences

Exon sequences (used for distance and Ks/Ka calculations)

Dotplots for pairs of genes in the family, including DNA, peptides, 5' and 3' ends


Gene family description for famliy 1019

Cytochrome P450

EST Information
Gene ESTs exist? ESTs confirm introns?
at3g56630 no --
at1g34540 no --

Genetic distances | Percent peptide similarity (upper) and Ks/Ka ratios (lower)
-- at3g56630 at1g34540
at3g56630 -- 9.91 | 88.6%
at1g34540 3.792 --

Aligned DNA and peptide sequences

Exon sequences (used for distance and Ks/Ka calculations)

Dotplots for pairs of genes in the family, including DNA, peptides, 5' and 3' ends


Gene family description for famliy 1004

Alpha-galactosyl transferase

EST Information
Gene ESTs exist? ESTs confirm introns?
at4g02500 yes no
at3g62720 yes --

Genetic distances | Percent peptide similarity (upper) and Ks/Ka ratios (lower)
-- at4g02500 at3g62720
at4g02500 -- 26.39 | 84.0%
at3g62720 11.225 --

Aligned DNA and peptide sequences

Exon sequences (used for distance and Ks/Ka calculations)

Dotplots for pairs of genes in the family, including DNA, peptides, 5' and 3' ends


Gene family description for famliy 1003

Beta-ketoacyl-CoA synthase

EST Information
Gene ESTs exist? ESTs confirm introns?
at3g10280 no --
at2g46720 yes --

Genetic distances | Percent peptide similarity (upper) and Ks/Ka ratios (lower)
-- at3g10280 at2g46720
at3g10280 -- 1.08 | 99.1%
at2g46720 8.718 --

Aligned DNA and peptide sequences

Exon sequences (used for distance and Ks/Ka calculations)

Dotplots for pairs of genes in the family, including DNA, peptides, 5' and 3' ends


Gene family description for famliy 899

Nodulin-like protein

EST Information
Gene ESTs exist? ESTs confirm introns?
at2g39210 no --
at2g28120 yes --

Genetic distances | Percent peptide similarity (upper) and Ks/Ka ratios (lower)
-- at2g39210 at2g28120
at2g39210 -- 53.83 | 61.6%
at2g28120 6.763 --

Aligned DNA and peptide sequences

Exon sequences (used for distance and Ks/Ka calculations)

Dotplots for pairs of genes in the family, including DNA, peptides, 5' and 3' ends


Gene family description for famliy 894

Putative protein

EST Information
Gene ESTs exist? ESTs confirm introns?
at4g05540 no --
at5g52090 weak --

Genetic distances | Percent peptide similarity (upper) and Ks/Ka ratios (lower)
-- at4g05540 at5g52090
at4g05540 -- 28.63 | 65.3%
at5g52090 1.383 --

Aligned DNA and peptide sequences

Exon sequences (used for distance and Ks/Ka calculations)

Dotplots for pairs of genes in the family, including DNA, peptides, 5' and 3' ends


Gene family description for famliy 591

Threonine synthase

EST Information
Gene ESTs exist? ESTs confirm introns?
at1g72810 yes no
at4g29840 yes --

Genetic distances | Percent peptide similarity (upper) and Ks/Ka ratios (lower)
-- at1g72810 at4g29840
at1g72810 -- 35.30 | 79.8%
at4g29840 9.129 --

Aligned DNA and peptide sequences

Exon sequences (used for distance and Ks/Ka calculations)

Dotplots for pairs of genes in the family, including DNA, peptides, 5' and 3' ends


Gene family description for famliy 565

Amino acid transporter

EST Information
Gene ESTs exist? ESTs confirm introns?
at4g21120 no --
at1g17120 yes --
at2g34960 no --

Genetic distances | Percent peptide similarity (upper) and Ks/Ka ratios (lower)
-- at4g21120 at1g17120 at2g34960
at4g21120 -- 78.97 | 54.3% 71.15 | 61.5%
at1g17120 ND -- 50.81 | 60.7%
at2g34960 ND ND --

Aligned DNA and peptide sequences

Exon sequences (used for distance and Ks/Ka calculations)

Dotplots for pairs of genes in the family, including DNA, peptides, 5' and 3' ends


Gene family description for famliy 335

Dimethylanaline monooxygenase

EST Information
Gene ESTs exist? ESTs confirm introns?
at1g04180 yes no
at4g28720 no --
at5g43890 no --

Genetic distances | Percent peptide similarity (upper) and Ks/Ka ratios (lower)
-- at1g04180 at4g28720 at5g43890
at1g04180 -- 45.67 | 73.6% 22.70 | 83.9%
at4g28720 5.853 -- 47.41 | 74.8%
at5g43890 9.118 5.970 --

Aligned DNA and peptide sequences

Exon sequences (used for distance and Ks/Ka calculations)

Dotplots for pairs of genes in the family, including DNA, peptides, 5' and 3' ends


Gene family description for famliy 246

Pyruvate decarboxylase

EST Information
Gene ESTs exist? ESTs confirm introns?
at5g01320 no --
at5g01330 no --
at4g33070 yes some
at5g54960 yes --

Genetic distances | Percent peptide similarity (upper) and Ks/Ka ratios (lower)
-- at5g01320 at5g01330 at4g33070 at5g54960
at5g01320 -- 7.31 | 92.5% 14.16 | 89.2% 28.42 | 81.6%
at5g01330 5.465 -- 15.01 | 87.0% 30.35 | 79.9%
at4g33070 8.511 5.921 -- 29.68 | 82.2%
at5g54960 10.179 10.185 11.650 --

Aligned DNA and peptide sequences

Exon sequences (used for distance and Ks/Ka calculations)

Dotplots for pairs of genes in the family, including DNA, peptides, 5' and 3' ends


Gene family description for famliy 218

Cytochrome P450

EST Information
Gene ESTs exist? ESTs confirm introns?
at1g01600 yes yes
at4g00360 yes yes
at2g45970 weak --
at1g63710 yes --

Genetic distances | Percent peptide similarity (upper) and Ks/Ka ratios (lower)
-- at1g01600 at4g00360 at2g45970 at1g63710
at1g01600 -- 18.61 | 88.8% 31.61 | 77.0% 39.64 | 70.3%
at4g00360 10.859 -- 31.32 | 76.6% 41.72 | 70.5%
at2g45970 7.255 6.363 -- 39.10 | 71.2%
at1g63710 5.423 6.908 6.151 --

Aligned DNA and peptide sequences

Exon sequences (used for distance and Ks/Ka calculations)

Dotplots for pairs of genes in the family, including DNA, peptides, 5' and 3' ends


Gene family description for famliy 179

Beta-glucosidase

EST Information
Gene ESTs exist? ESTs confirm introns?
at2g44450 yes some
at3g60130 yes some
at2g25630 yes --
at5g44640 yes --
at5g42260 no --

Genetic distances | Percent peptide similarity (upper) and Ks/Ka ratios (lower)
-- at2g44450 at3g60130 at2g25630 at5g44640 at5g42260
at2g44450 -- 30.36 | 71.2% 14.57 | 89.1% 14.14 | 89.3% 14.62 | 88.6%
at3g60130 4.457 -- 31.48 | 71.3% 30.68 | 73.0% 31.08 | 73.2%
at2g25630 7.211 5.259 -- 12.80 | 93.8% 12.96 | 92.5%
at5g44640 8.047 5.082 9.724 -- 3.97 | 96.3%
at5g42260 7.862 5.205 8.717 5.330 --

Aligned DNA and peptide sequences

Exon sequences (used for distance and Ks/Ka calculations)

Dotplots for pairs of genes in the family, including DNA, peptides, 5' and 3' ends


Gene family description for famliy 152

Hypothetical or putative protein

EST Information
Gene ESTs exist? ESTs confirm introns?
at5g45530 no --
at5g45470 yes --
at5g45460 yes --
at5g45480 yes --
at5g45540 no --

Genetic distances | Percent peptide similarity (upper) and Ks/Ka ratios (lower)
-- at5g45530 at5g45470 at5g45460 at5g45480 at5g45540
at5g45530 -- 54.23 | 58.0% -- | 52.1% 64.92 | 43.3% 57.99 | 52.3%
at5g45470 6.079 -- -- | 77.7% 60.13 | 42.1% 58.41 | 50.3%
at5g45460 -- -- -- -- | 44.1% -- | 43.0%
at5g45480 ND 3.341 -- -- 44.24 | 60.1%
at5g45540 4.642 3.934 -- 3.881 --

Aligned DNA and peptide sequences

Exon sequences (used for distance and Ks/Ka calculations)

Dotplots for pairs of genes in the family, including DNA, peptides, 5' and 3' ends


Gene family description for famliy 142

CDC 20 or WD-repeat protein

EST Information
Gene ESTs exist? ESTs confirm introns?
at4g33260 yes some
at4g33270 yes some
at5g27080 no --
at5g26900 no --
at5g27570 no --

Genetic distances | Percent peptide similarity (upper) and Ks/Ka ratios (lower)
-- at4g33260 at4g33270 at5g27080 at5g26900 at5g27570
at4g33260 -- 2.53 | 98.7% 19.49 | 78.5% 19.59 | 77.6% -- | 80.2%
at4g33270 12.781 -- 19.19 | 78.0% 19.29 | 76.8% -- | 80.5%
at5g27080 3.295 3.280 -- 2.71 | 93.7% -- | 92.0%
at5g26900 2.965 2.940 1.503 -- -- | 91.2%
at5g27570 -- -- -- -- --

Aligned DNA and peptide sequences

Exon sequences (used for distance and Ks/Ka calculations)

Dotplots for pairs of genes in the family, including DNA, peptides, 5' and 3' ends


Gene family description for famliy 137

AMP-binding protein

EST Information
Gene ESTs exist? ESTs confirm introns?
at1g21530 no --
at1g21540 yes no
at5g16340 no --
at5g16370 yes --
at1g75960 no --
at1g77240 yes --

Genetic distances | Percent peptide similarity (upper) and Ks/Ka ratios (lower)
-- at1g21530 at1g21540 at5g16340 at5g16370 at1g75960 at1g77240
at1g21530 -- 15.82 | 89.4% 46.90 | 62.3% 47.60 | 61.2% 51.05 | 60.8% 28.03 | 59.8%
at1g21540 8.671 -- 47.94 | 62.2% 48.52 | 61.7% 54.01 | 60.5% 26.44 | 80.0%
at5g16340 4.575 4.983 -- 6.85 | 91.8% 22.56 | 79.6% 51.12 | 59.3%
at5g16370 4.343 5.123 4.504 -- 23.48 | 79.8% 52.36 | 60.2%
at1g75960 4.641 5.648 4.776 5.094 -- 54.40 | 59.8%
at1g77240 7.803 7.919 5.756 6.822 5.564 --

Aligned DNA and peptide sequences

Exon sequences (used for distance and Ks/Ka calculations)

Dotplots for pairs of genes in the family, including DNA, peptides, 5' and 3' ends


Gene family description for famliy 111

Inorganic phosphate transporter

EST Information
Gene ESTs exist? ESTs confirm introns?
at2g32830 no --
at5g43350 yes no
at5g43360 no --
at5g43370 yes yes
at5g43340 weak --
at2g38940 yes --
at3g54700 weak --

Genetic distances | Percent peptide similarity (upper) and Ks/Ka ratios (lower)
-- at2g32830 at5g43350 at5g43360 at5g43370 at5g43340 at2g38940 at3g54700
at2g32830 -- 43.39 | 76.0% 45.37 | 77.2% 43.04 | 75.2% 55.13 | 65.3% 42.36 | 75.9% 41.33 | 75.9%
at5g43350 10.541 -- 10.67 | 93.7% 2.30 | 98.9% 51.68 | 67.8% 37.09 | 77.7% 37.04 | 78.3%
at5g43360 ND 10.046 -- 11.35 | 93.5% 51.42 | 68.8% 35.52 | 78.6% 36.30 | 79.3%
at5g43370 9.541 10.286 10.089 -- 51.42 | 67.7% 36.46 | 77.7% 36.62 | 78.1%
at5g43340 4.461 ND ND ND -- 50.77 | 67.0% 46.34 | 69.1%
at2g38940 12.176 12.862 12.111 12.830 ND -- 20.41 | 88.7%
at3g54700 12.912 11.457 16.432 11.632 6.028 15.087 --

Aligned DNA and peptide sequences

Exon sequences (used for distance and Ks/Ka calculations)

Dotplots for pairs of genes in the family, including DNA, peptides, 5' and 3' ends


Gene family description for famliy 88

Subtilisin-like serine proteinase

EST Information
Gene ESTs exist? ESTs confirm introns?
at1g01900 yes no
at2g05920 yes yes
at1g04110 no --
at5g51750 yes --
at3g14240 yes --
at4g34980 yes --
at5g67360 yes --

Genetic distances | Percent peptide similarity (upper) and Ks/Ka ratios (lower)
-- at1g01900 at2g05920 at1g04110 at5g51750 at3g14240 at4g34980 at5g67360
at1g01900 -- 78.74 | 45.8% 81.07 | 43.9% 78.75 | 45.6% 77.82 | 44.7% 76.80 | 45.0% 73.44 | 46.8%
at2g05920 4.670 -- 73.44 | 48.7% 69.99 | 49.6% 67.24 | 50.5% 69.35 | 49.3% 62.37 | 55.6%
at1g04110 ND 4.940 -- 78.30 | 43.2% 76.67 | 44.7% 77.44 | 43.5% 75.02 | 46.1%
at5g51750 ND 5.101 5.258 -- 72.45 | 47.7% 70.62 | 49.4% 64.64 | 53.1%
at3g14240 5.029 3.243 ND 5.761 -- 58.54 | 56.1% 63.13 | 51.8%
at4g34980 3.618 5.329 ND 5.222 4.010 -- 63.10 | 51.1%
at5g67360 4.065 4.022 3.695 4.802 3.431 3.677 --

Aligned DNA and peptide sequences

Exon sequences (used for distance and Ks/Ka calculations)

Dotplots for pairs of genes in the family, including DNA, peptides, 5' and 3' ends


Gene family description for famliy 76

Pyruvate kinase

EST Information
Gene ESTs exist? ESTs confirm introns?
at5g56350 yes yes
at4g26390 no --
at5g63680 yes yes
at5g08570 yes yes
at3g04050 no --
at3g55650 no --
at3g55810 no --
at3g25960 no --

Genetic distances | Percent peptide similarity (upper) and Ks/Ka ratios (lower)
-- at5g56350 at4g26390 at5g63680 at5g08570 at3g04050 at3g55650 at3g55810 at3g25960
at5g56350 -- 20.77 | 90.4% 34.48 | 79.0% 36.31 | 79.0% 41.71 | 73.0% 41.83 | 71.5% 42.10 | 70.9% 40.35 | 73.9%
at4g26390 18.943 -- 38.29 | 77.1% 40.10 | 76.7% 42.06 | 72.3% 43.60 | 69.1% 43.64 | 69.0% 43.96 | 71.6%
at5g63680 10.733 10.943 -- 16.73 | 92.8% 44.33 | 69.4% 45.18 | 68.0% 44.85 | 67.3% 42.86 | 70.5%
at5g08570 10.157 11.304 15.247 -- 45.54 | 69.2% 46.41 | 68.0% 46.08 | 67.3% 45.45 | 69.9%
at3g04050 8.787 8.526 8.484 9.707 -- 17.41 | 84.9% 17.42 | 84.6% 18.54 | 86.5%
at3g55650 7.383 6.948 8.193 10.983 5.653 -- 2.02 | 97.2% 8.28 | 91.6%
at3g55810 7.802 7.459 8.342 ND 6.116 4.219 -- 8.13 | 91.7%
at3g25960 7.000 8.901 8.398 10.362 8.159 4.594 5.443 --

Aligned DNA and peptide sequences

Exon sequences (used for distance and Ks/Ka calculations)

Dotplots for pairs of genes in the family, including DNA, peptides, 5' and 3' ends


Gene family description for famliy 69

Heat shock protein hsp70

EST Information
Gene ESTs exist? ESTs confirm introns?
at5g28540 yes some
at5g42020 yes some
at1g09080 no --
at1g56410 no --
at3g09440 yes yes
at3g12580 yes no
at5g02490 yes no
at5g02500 yes no
at1g16030 yes --

Genetic distances | Percent peptide similarity (upper) and Ks/Ka ratios (lower)
-- at5g28540 at5g42020 at1g09080 at1g56410 at3g09440 at3g12580 at5g02490 at5g02500 at1g16030
at5g28540 -- 2.36 | 98.8% 34.43 | 77.4% 48.09 | 62.3% 46.15 | 64.0% 46.96 | 62.9% 47.47 | 62.9% 47.16 | 62.8% 51.02 | 62.8%
at5g42020 18.314 -- 35.29 | 77.2% 47.78 | 62.0% 45.16 | 63.8% 47.87 | 62.5% 47.16 | 62.7% 46.66 | 62.5% 51.13 | 62.5%
at1g09080 12.583 13.473 -- 52.01 | 60.1% 49.96 | 61.8% 50.80 | 61.2% 51.99 | 59.8% 51.48 | 60.5% 49.86 | 61.9%
at1g56410 5.042 5.003 7.248 -- 21.03 | 87.5% 26.28 | 86.9% 13.43 | 89.6% 18.36 | 89.3% 33.91 | 79.5%
at3g09440 4.660 4.448 7.722 12.285 -- 23.48 | 90.9% 17.96 | 91.7% 17.21 | 93.1% 31.83 | 81.7%
at3g12580 5.904 5.866 8.716 12.047 20.412 -- 23.48 | 90.2% 23.33 | 91.2% 28.40 | 84.5%
at5g02490 5.133 5.047 8.993 7.051 18.321 17.458 -- 14.22 | 94.2% 31.28 | 81.0%
at5g02500 4.904 4.740 7.622 11.192 19.371 19.694 15.272 -- 32.35 | 81.9%
at1g16030 7.085 6.601 5.960 12.415 13.285 15.672 15.142 15.142 --

Aligned DNA and peptide sequences

Exon sequences (used for distance and Ks/Ka calculations)

Dotplots for pairs of genes in the family, including DNA, peptides, 5' and 3' ends


Gene family description for famliy 54

Hypothetical CHP-rich zinc finger protein

EST Information
Gene ESTs exist? ESTs confirm introns?
at5g42280 no --
at1g55410 no --
at1g55380 no --
at1g55390 weak --
at1g55420 weak --
at1g55430 no --
at1g55440 no --

Genetic distances | Percent peptide similarity (upper) and Ks/Ka ratios (lower)
-- at5g42280 at1g55410 at1g55380 at1g55390 at1g55420 at1g55430 at1g55440
at5g42280 -- 23.50 | 64.9% 22.03 | 70.2% 21.60 | 67.9% 21.76 | 66.8% 23.31 | 64.2% 24.50 | 65.8%
at1g55410 1.872 -- 24.24 | 70.2% 24.37 | 67.6% 24.16 | 66.7% 26.37 | 64.2% 27.02 | 65.2%
at1g55380 2.175 1.881 -- 20.46 | 70.6% 8.41 | 81.8% 18.99 | 71.8% 23.89 | 66.8%
at1g55390 2.183 1.811 2.142 -- 21.21 | 66.9% 22.40 | 66.9% 22.98 | 67.6%
at1g55420 2.045 1.817 2.181 2.049 -- 18.27 | 69.4% 23.76 | 65.9%
at1g55430 1.604 1.373 1.481 1.486 1.366 -- 25.06 | 64.1%
at1g55440 1.631 1.483 1.664 1.613 1.687 1.241 --

Aligned DNA and peptide sequences

Exon sequences (used for distance and Ks/Ka calculations)

Dotplots for pairs of genes in the family, including DNA, peptides, 5' and 3' ends


Gene family description for famliy 39

Fatty acid elongase

EST Information
Gene ESTs exist? ESTs confirm introns?
at1g04220 yes yes
at5g43760 yes yes
at1g25450 yes yes
at1g68530 yes yes
at1g01120 yes --
at1g19440 yes --
at2g15090 yes --
at2g16280 yes --
at2g26640 yes --
at4g34250 yes --
at4g34510 yes --
at4g34520 yes --

Genetic distances | Percent peptide similarity (upper) and Ks/Ka ratios (lower)
-- at1g04220 at5g43760 at1g25450 at1g68530 at1g01120 at1g19440 at2g15090 at2g16280 at2g26640 at4g34250 at4g34510 at4g34520
at1g04220 -- 19.76 | 88.1% 60.22 | 57.5% 57.10 | 57.7% 53.19 | 58.9% 54.40 | 62.3% 57.01 | 57.4% 52.51 | 62.8% 44.08 | 72.2% 58.02 | 54.9% 51.14 | 61.3% 57.37 | 52.6%
at5g43760 9.170 -- 56.92 | 58.9% 54.96 | 58.5% 54.04 | 59.5% 52.77 | 62.7% 53.25 | 57.6% 51.21 | 62.1% 42.19 | 70.7% 56.46 | 54.9% 49.44 | 59.5% 55.98 | 54.9%
at1g25450 7.117 6.400 -- 21.27 | 88.6% 62.93 | 55.9% 54.82 | 61.5% 61.39 | 54.0% 53.21 | 62.3% 62.28 | 57.3% 64.77 | 50.9% 54.49 | 57.4% 63.93 | 50.8%
at1g68530 ND 5.915 14.994 -- 63.10 | 55.5% 55.26 | 61.9% 64.70 | 54.2% 54.67 | 62.7% 61.63 | 57.3% 65.79 | 51.4% 58.62 | 58.3% 66.48 | 51.7%
at1g01120 5.225 6.493 5.832 ND -- 59.86 | 57.5% 60.38 | 54.2% 58.30 | 55.3% 58.11 | 61.0% 63.93 | 51.7% 60.86 | 55.0% 63.76 | 51.6%
at1g19440 7.721 10.133 7.305 8.866 ND -- 47.37 | 63.9% 31.16 | 76.2% 50.64 | 65.3% 47.90 | 62.7% 37.16 | 70.8% 46.30 | 63.8%
at2g15090 6.063 4.326 6.044 ND 6.164 7.121 -- 43.70 | 62.6% 59.31 | 57.4% 27.30 | 75.7% 43.05 | 61.8% 43.08 | 65.5%
at2g16280 6.418 7.155 ND ND ND 8.233 5.519 -- 48.98 | 63.9% 42.94 | 61.4% 25.27 | 79.7% 39.61 | 66.7%
at2g26640 11.625 9.749 ND ND ND 9.521 ND 7.089 -- 60.98 | 55.9% 51.72 | 60.3% 59.86 | 56.0%
at4g34250 5.043 4.151 ND ND 5.185 6.533 5.162 4.815 ND -- 44.47 | 61.2% 40.74 | 64.8%
at4g34510 5.899 5.335 ND ND 7.169 7.695 4.778 6.428 5.515 4.382 -- 39.81 | 64.2%
at4g34520 5.511 9.170 ND ND 4.316 8.241 5.153 5.544 7.413 4.490 4.880 --

Aligned DNA and peptide sequences

Exon sequences (used for distance and Ks/Ka calculations)

Dotplots for pairs of genes in the family, including DNA, peptides, 5' and 3' ends


Gene family description for famliy 36

Receptor protein kinase

EST Information
Gene ESTs exist? ESTs confirm introns?
at1g70110 no --
at1g70130 no --
at2g37710 yes --
at2g43690 no --
at2g43700 no --
at3g55550 no --
at3g59700 yes --
at3g59730 no --
at3g59740 yes --
at3g59750 no --
at4g02410 no --
at4g02420 weak --
at4g29050 yes --

Genetic distances | Percent peptide similarity (upper) and Ks/Ka ratios (lower)
-- at1g70110 at1g70130 at2g37710 at2g43690 at2g43700 at2g59730 at3g55550 at3g59700 at3g59740 at3g59750 at4g02410 at4g02420 at4g29050
at1g70110 -- 40.06 | 61.5% 72.18 | 46.8% 60.96 | 49.8% 60.01 | 51.7% -- | 46.5% 73.90 | 43.2% 61.23 | 52.3% 61.81 | 50.6% 60.37 | 50.3% 71.75 | 44.0% 75.49 | 46.5% 32.95 | 69.4%
at1g70130 3.458 -- 73.89 | 44.0% 66.57 | 46.3% 64.27 | 49.0% -- | 47.4% 78.30 | 42.3% 64.47 | 48.1% 66.87 | 47.0% 67.27 | 46.5% 73.45 | 43.1% 75.35 | 43.2% 37.42 | 65.3%
at2g37710 ND 4.943 -- 75.904 | 42.7% 73.09 | 44.7% -- | 46.5% 69.94 | 48.5% 73.92 | 45.2% 77.47 | 43.7% 76.41 | 42.4% 26.69 | 73.6% 26.62 | 76.8% 70.49 | 48.9%
at2g43690 3.882 3.611 4.753 -- 26.57 | 70.5% -- | 64.5% 80.16 | 42.0% 41.70 | 62.2% 42.43 | 60.5% 41.17 | 60.5% 75.29 | 41.3% 75.59 | 42.1% 57.09 | 50.5%
at2g43700 4.840 4.917 4.432 3.185 -- -- | 66.5% 77.56 | 43.5% 38.61 | 67.5% 39.22 | 65.3% 39.39 | 64.1% 72.02 | 44.0% 72.34 | 45.3% 58.64 | 52.7%
at2g59730 -- -- -- -- -- -- -- | 45.4% -- | 79.1% -- | 78.3% -- | 77.7% -- | 44.4% -- | 46.1% -- | 52.8%
at3g55550 4.402 4.115 4.868 4.699 4.877 -- -- 79.55 | 43.0% 78.43 | 42.8% 78.54 | 42.3% 69.96 | 47.2% 71.15 | 48.9% 75.31 | 45.6%
at3g59700 4.436 3.658 3.811 4.024 5.059 -- 7.743 -- 17.01 | 78.9% 19.19 | 77.0% 73.04 | 44.1% 74.67 | 44.5% 56.34 | 53.0%
at3g59740 4.601 3.811 3.960 3.533 4.135 -- ND 2.344 -- 14.89 | 82.1% 75.29 | 43.4% 76.24 | 44.0% 57.90 | 51.6%
at3g59750 4.054 3.769 3.519 3.738 4.044 -- 5.292 2.529 2.431 -- 74.66 | 42.3% 74.73 | 43.1% 58.61 | 51.6%
at4g02410 ND 4.060 3.898 6.348 4.596 -- 5.000 3.990 4.289 3.826 -- 19.27 | 78.0% 69.21 | 47.5%
at4g02420 ND 5.958 4.507 5.218 ND -- 5.288 6.174 5.181 4.029 3.561 -- 69.23 | 49.1%
at4g29050 3.479 3.681 7.658 3.437 4.941 -- 4.723 4.125 4.447 4.257 6.326 7.382 --

Aligned DNA and peptide sequences

Exon sequences (used for distance and Ks/Ka calculations)

Dotplots for pairs of genes in the family, including DNA, peptides, 5' and 3' ends